Products that can be tested with ISO 18184
The ISO 18184 can be used to evaluate the antiviral activity of different textile products such as
woven, non-woven & knitted fabrics,
yarns,
active wear,
socks
daily wear,
health care products such as scrubs, masks, & surgical clothes,
and other home textiles.
ISO 18184 is used for textile products that are hydrophilic in nature. For hydrophobic textiles, ISO 21702 shall be used to evaluate antiviral activity.
Sample Preparation
Control Sample – 9 sterilized control (untreated) specimens with a mass of 0.4g ± 0.05g are required for ISO 18184 testing.
3 control specimens are used to determine the infectious titre of the virus immediately after inoculation.
3 control specimens are used to determine the infectious titre of the remaining virus after inoculation for a “contact time” (Standard “contact time” is 2 hours, but can go up to 24 hours).
3 control specimens are used for cytotoxicity analysis.
Test samples – 6 sterilized test (treated) samples with a mass of 0.4g ± 0.05g are required for ISO 18184 testing.
3 treated specimens to determine the infectious titre of the remaining virus after inoculation is in contact with the treated specimen same as the control specimen.
3 treated specimens are used for cytotoxicity analysis.
We test using following organisms: SARS CoV-2, Beta Coronavirus (OC-43) (ATCC VR-1558) , Human Coronavirus (229E) (ATCC VR-740) , Influenza A (H1N1) (ATCC VR-1469) Influenza A (H3N2) (ATCC VR-1679), Adenovirus, Norovirus, Poliovirus, Vaccinia virus, Feline Calcivirus
ISO 18184 Test Procedure
Control and treated samples are placed in separate sterile plates.
The 200 uL of the virus is inoculated on both the control and the treated samples.
Immediately after the inoculation of the virus, 20 ml of SCDLP ( used as a neutralizing solution) is added to 3 control samples.
After a specific contact period, 20 ml of SCDLP broth is added to 3 treated and 3 untreated samples to recover the remaining virus.
The wash-out solution is serially diluted up to 10 dilutions, and the infectious titre of the recovered virus is determined either by Plaque assay or by TCID50 assay. Other assays can also be used based on the virus strain.
Antiviral Activity and Performance Standard
The antiviral activity is determined by the following equation.
Mv = Log10 (Va) – Log10 (Vc)
Where,
Mv is the antiviral activity value
Log10 (Va) is the logarithm average of 3 infectivity titre value immediately after inoculation of the control specimen
Log10 (Vc) is the logarithm average of 3 infectivity titre value after specific contact time with the test specimen.
As per the ISO 18184, If the log value is between 2 and 3, the antiviral performance of the textile product is considered good. If the log value is greater than or equal to 3, then the antiviral performance of textile product is considered excellent.
Antiviral activity value (Mv) | Efficacy Rating |
2 ≤ Mv < 3 | Good Effect Level |
Mv ≥ 3 | Excellent Effect Level |
Table 1: Antiviral performance standard
The ISO 18184 is a very robust test method. If followed properly, the results are accurate and repeatable in both inter laboratory testing and intra laboratory testing. In the ISO 18184 test protocol, the steps are given for Influenza A (H3N2, and H1N1), and Feline calcivirus, but the protocol can be used for other viruses also after appropriate validations.
At Feifan, we evaluate the efficacy of a wide range of antimicrobial textile materials such as woven, non-woven & knitted fabrics and textile clothes used in health care settings.
We measure the antiviral activity on textile materials using standard methods like JIS L 1922 and ISO 18184. While, ISO 20743, JIS L 1902, and AATCC 147 are standard protocols used for assessing the antibacterial activity of textile materials.
We also offer a comprehensive array of testing services to determine the antimicrobial efficacy of plastics, coatings, and surface disinfectants.
To request a quote or for more information on ISO 18184 testing, get a free consultation from our experts.
ISO 18184 Test Procedure
Control and treated samples are placed in separate sterile plates.
The 200 uL of the virus is inoculated on both the control and the treated samples.
Immediately after the inoculation of the virus, 20 ml of SCDLP ( used as a neutralizing solution) is added to 3 control samples.
After a specific contact period, 20 ml of SCDLP broth is added to 3 treated and 3 untreated samples to recover the remaining virus.
The wash-out solution is serially diluted up to 10 dilutions, and the infectious titre of the recovered virus is determined either by Plaque assay or by TCID50 assay. Other assays can also be used based on the virus strain.
Antiviral Activity and Performance Standard
The antiviral activity is determined by the following equation.
Mv = Log10 (Va) – Log10 (Vc)
Where,
Mv is the antiviral activity value
Log10 (Va) is the logarithm average of 3 infectivity titre value immediately after inoculation of the control specimen
Log10 (Vc) is the logarithm average of 3 infectivity titre value after specific contact time with the test specimen.
As per the ISO 18184, If the log value is between 2 and 3, the antiviral performance of the textile product is considered good. If the log value is greater than or equal to 3, then the antiviral performance of textile product is considered excellent.
Antiviral activity value (Mv) | Efficacy Rating |
2 ≤ Mv < 3 | Good Effect Level |
Mv ≥ 3 | Excellent Effect Level |
Table 1: Antiviral performance standard
The ISO 18184 is a very robust test method. If followed properly, the results are accurate and repeatable in both inter laboratory testing and intra laboratory testing. In the ISO 18184 test protocol, the steps are given for Influenza A (H3N2, and H1N1), and Feline calcivirus, but the protocol can be used for other viruses also after appropriate validations.
At Feifan, we evaluate the efficacy of a wide range of antimicrobial textile materials such as woven, non-woven & knitted fabrics and textile clothes used in health care settings.
We measure the antiviral activity on textile materials using standard methods like JIS L 1922 and ISO 18184. While, ISO 20743, JIS L 1902, and AATCC 147 are standard protocols used for assessing the antibacterial activity of textile materials.
We also offer a comprehensive array of testing services to determine the antimicrobial efficacy of plastics, coatings, and surface disinfectants.
To request a quote or for more information on ISO 18184 testing, get a free consultation from our experts.
